AltoStar® Purification Kit 1.5 RUO

 

The AltoStar® Purification Kit 1.5 uses magnetic particle technology and allows the isolation and purification of nucleic acids from biological specimens in conjunction with the AltoStar® Automation System AM16 (Hamilton).

 

AltoStar® Purification Kit 1.5 RUO — Delivery of two boxes

For research use only (RUO). Not for use in diagnostic procedures.

Key features

  • DNA and RNA purification in parallel
  • All sample types in one run
  • Analyze up to four pathogens from a single sample

Sample types

The following sample types can be used with the AltoStar® Purification Kit 1.5:

 

Whole blood

Serum

Plasma

Urine

Stool

Cerebrospinal fluid (CSF)

Swabs in transport medium

Kit content

 

All reagents included in the AltoStar® Purification Kit 1.5 are ready-to-use solutions and are shipped in two separate boxes:

Box 1 and Box 2. The boxes are shipped at room temperature.

 

Box 1 (with 6 different boxes inside) has to be stored at +15 °C to +30 °C and

 

Box 2 (with 6 trays inside) has to be stored at +2 °C to +8 °C upon receipt.

 

The AltoStar® Purification Kit 1.5 contains reagents sufficient for 1152 sample purifications with a 500 µl sample volume, or for 576 sample purifications with a 1000 µl sample volume.

AltoStar® Purification Kit 1.5 RUO — Content of Box 1 (with 6 different boxes inside)

AltoStar® Purification Kit 1.5 RUO — Content of Box 2 (6 trays with tubes inside)

AltoStar® Purification Kit 1.5 RUO

Order No.PK15-06
Sample purifications1152 (500 µl sample volume, low volume) or 576 (1000 µl sample volume, high volume)
TransportationRoom temperature
Can be used withAltoStar® Automation System AM16

AltoStar® Purification Kit 1.5 RUO

Instructions for use AltoStar® Purification Kit 1.5

pdf
File size: 1.38 MB
Release date: 05/2022

Principle of method

The AltoStar® Purification Kit 1.5 is intended for automated isolation and purification of RNA and DNA from specified biological specimens for in vitro diagnostic purposes in conjunction with the AltoStar® AM16. The AltoStar® Purification Kit 1.5 is based on magnetic bead technology, utilizing silica-coated magnetic particles, which can bind and release nucleic acids under specific conditions.

 

The purification procedure comprises 3 automated steps on the AltoStar® AM16 (see Fig.)

Sample lysis and DNA/RNA binding

1. In the first step nucleic acids are released by chemical and mechanical lysis under chaotropic high salt conditions. The conditions stabilize the nucleic acids in solution and enable their binding to the magnetic silica beads. The AltoStar® Internal Control 1.5 is automatically added by the AltoStar® AM16.